John B. McCullough

John B. McCullough is a biochemistry researcher specializing in structural biology and membrane remodeling, with a focus on the ESCRT-III family of proteins. His work combines cryogenic electron microscopy (cryo-EM) and biochemical analysis to investigate protein interactions critical to HIV infection and viral dynamics.

Research Highlights:

• First high-resolution structure of an ESCRT-III protofilament
• Co-first author on a novel ESCRT-III/DNA bridge association
• Scientific manager of the NIAID-funded U54 CHEETAH Center

Key Research Areas:

• ESCRT-III structure and function
• Membrane remodeling and scission
• Cryo-EM techniques
• HIV viral dynamics

Selected Trends (2018–2025):

• ESCRT-III/nucleic acid interactions
• Thylakoid membrane remodeling
• Microfluidic device applications in lipid vesicle studies
• HIV budding and restriction mechanisms