Marcelo B. Sztein, MD is a tenured Professor of Pediatrics at the University of Maryland School of Medicine (UMSOM). Since 1989 he has been a key leader within the Center for Vaccine Development (CVD) , serving as Chief of the Cellular Immunology and Flow Cytometry Section, founder of the CVD Immunology Group, and—since 2010— Associate Director for Immunologic Research . He holds secondary appointments in the Departments of Medicine and Microbiology & Immunology and directs the CVD Flow & Mass Cytometry Core that supports campus-wide cutting-edge single-cell analysis. Education & Training High School #4, Nicolás Avellaneda, Argentina — B.S., Biology, 1970 University of Buenos Aires School of Medicine — M.D., 1976 National Research Council, Argentina — Fellow, Immunology, 1976–1979 National Institutes of Health — Visiting Fellow, 1979–1982 George Washington University Medical Center — Research Fellow, Cancer Research Laboratories, 1982–1983 Research Focus Dr. Sztein is an internationally recognized authority on the immunology of infectious diseases , with emphasis on mucosal and systemic immunity elicited by vaccines. His work deciphers human host responses to Salmonella Typhi/Paratyphi, Shigella , ETEC, Plasmodium falciparum , dengue virus, Ebola virus, H. pylori , and influenza. Using controlled human infection models, non-human primates, and novel organotypic intestinal models, his laboratory identifies correlates of protection, dissects multifunctional T-cell subsets (CD4⁺, CD8⁺, MAIT, regulatory T cells), and explores how the gut microbiome modulates vaccine-induced immunity. Current projects under the NIH-funded Cooperative Center for Human Immunology (CCHI) integrate multi-omics , single-cell mass cytometry (CyTOF) , and high-dimensional flow cytometry to accelerate rational vaccine design. Recent Publication Trends Between 2014 and 2016 Dr. Sztein’s group published >25 high-impact papers that collectively advance three major themes: (1) elucidating multifunctional T- and B-cell signatures associated with protection following typhoid and Shigella vaccination; (2) applying controlled human infection models to rigorously benchmark new vaccines; and (3) pioneering 3-D organotypic intestinal cultures and mass cytometry panels to study mucosal responses at single-cell resolution. These studies are shaping the next generation of enteric vaccines for global health. Grants & Funding Leadership Principal Investigator, NIH U19-AI082655 “Mucosal and Systemic Immunity, Vaccines and Microbiota Interplay in Humans” (2014–2019) Principal Investigator, NIH R01-AI036525 “Immune Mechanisms of Protection in S. Typhi Vaccines” (2013–2018) PI Research Project 1, NIH U19-AI109776 “Immunoprophylactic Strategies to Control Emerging Enteric Infections” (2014–2019) PI of Immunology Core, NIH U19-AI110820 “Host, Pathogen, and the Microbiome” (2014–2019) Senior Immunologist, VTEU Contract HHSN2722013000221 (2013–2023) Laboratory PI, HHSN27200012 & HHSN27200010 cytokine/immune-phenotyping service contracts Co-Director of Immunology, T32-AI07524 Training Grant (2013–2018) Laboratory & Core Facilities Dr. Sztein directs the CVD Flow and Mass Cytometry Core , housing state-of-the-art BD LSR-II, Beckman-Coulter Astrios EQ cell sorter, Fluidigm CyTOF 1, and CyTOF Helios instruments. The core supports up to 19-parameter cell sorting and 35-plus-parameter mass cytometry, enabling investigators across UMSOM and collaborating institutions to perform advanced single-cell multi-omics studies under BSL-2/3 containment.
