معرفی
Joseph T. P. Yeeles is a Research Professor at the MRC Laboratory of Molecular Biology (LMB), University of Cambridge, where he leads a research group investigating chromosome replication mechanisms and genome stability. His work centers on the eukaryotic replisome—a complex molecular machine assembled from over 30 gene products—and how it ensures faithful genome duplication despite inherent risks like DNA strand separation and replication fork stalling.
His research employs in vitro reconstitution with purified proteins, combined with genetic approaches and cell-free extracts, to dissect replisome biology. Key focus areas include replication fork dynamics, responses to DNA damage, and regulation of replisome assembly/disassembly. His lab has made seminal contributions to understanding leading-strand synthesis, translesion synthesis, and fork protection mechanisms.
Analysis of his 2015-2022 publications reveals a consistent emphasis on structural and mechanistic insights into eukaryotic DNA replication. His work bridges biochemistry, structural biology, and genetics, with recurring themes of replication fidelity, replisome architecture, and damage response pathways—most notably advancing in vitro reconstitution techniques for human replication systems.
Yeeles heads a multidisciplinary team including Anne Donaldson, Federico Fassetta, Emma Fletcher, Morgan Jones, Harry Orrin, Cristian Polo Rivera, Jonas Roske, and Eleanor Taylor-Cross. The group leverages cryo-EM, biochemical assays, and genetic models to investigate fundamental replication processes within a highly controlled experimental framework.
