معرفی
Letizia Barbieri is a Researcher at the Interuniversity Consortium for Magnetic Resonance of Metallo Proteins (CIRMMP) and the Magnetic Resonance Center, University of Florence, Italy. Her work focuses on advancing in-cell NMR techniques to study proteins in their native cellular environment. She specializes in developing protocols for high protein expression in human cells (e.g., HEK293T) and optimizing NMR bioreactors for extended experiments. Her research bridges structural biology, drug screening, and protein-metal interactions, with applications in understanding disease mechanisms and drug development.
Barbieri holds a Chemistry degree (2010) and an International PhD in Mechanistic and Structural Systems Biology (2014), both from the University of Florence. Her research interests include in-cell NMR methodologies, protein-protein interactions, and real-time monitoring of biological processes. She has pioneered approaches for studying protein maturation, redox regulation, and ligand binding in live cells.
Her publications highlight innovations in NMR techniques, such as fluorinated amino acid incorporation and high-field NMR applications. She collaborates on projects like the synthesis of isotopically labeled fluorotryptophan and the development of bioreactors to enhance experimental timelines. These advancements enable atomic-resolution insights into protein behavior under physiological conditions.
Barbieri’s work has been recognized through collaborations with institutions like the University of Oxford and the National Institute for Research in Radiation Protection (ENEA). Her lab focuses on translating NMR data into actionable insights for drug design and understanding protein dysfunction in diseases like ALS.
