معرفی
Kenji Murakami is an Associate Professor in the Department of Biochemistry & Biophysics at the Perelman School of Medicine, University of Pennsylvania. His research integrates structural biology and biochemical approaches to study fundamental molecular mechanisms in transcription and DNA repair.
Research in the Murakami Lab focuses on
- Structural basis of transcription initiation and regulation
- Mechanistic analysis of RNA polymerase II dynamics
- High-resolution cryo-EM studies of protein-DNA complexes
- Terpene biosynthesis catalytic cascades
- Assembly-line mechanisms in multi-enzyme complexes
The lab employs advanced techniques including
- Single-particle cryo-EM
- Cryo-electron tomography (cryo-ET)
- X-ray crystallography
- In vitro transcription reconstitution
- Protein engineering
Recent publications highlight structural analyses of transcription preinitiation complexes, Mediator kinase modules, and DNA repair machinery, with methodological innovations in cryo-EM data interpretation.
Laboratory facilities include:
- 363 Clinical Research Building space
- Yeast biochemistry infrastructure
- Cryo-EM dedicated computer clusters
- Fermenter systems for protein production
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