James Nuñez

James Nuñez is an Assistant Professor specializing in Biochemistry, Biophysics, and Structural Biology. His research focuses on epigenetic memory, CRISPR-based technologies, and DNA methylation biology. He develops programmable epigenome editing tools like CRISPRoff to study gene expression regulation and disease mechanisms.

Research interests include understanding how epigenetic marks are established and maintained in mammalian cells, particularly during development. He combines CRISPR screens, functional genomics, and biochemistry to explore these processes. His lab also investigates the role of DNA methylation defects in cancer and neurological disorders.

Recent work highlights CRISPR-based methods for transient epigenome editing, enabling reversible gene silencing without DNA breaks. These tools are applied to cell engineering and therapeutic development. His lab maintains a focus on translating basic research into clinical applications.

Major contributions include foundational studies on CRISPR-Cas systems and the development of novel epigenetic editing platforms. The lab actively collaborates on projects involving computational imaging and multi-omics analysis to further dissect complex biological systems.