معرفی
Dr Greg H. P. Ngo is a Research Fellow at Cardiff University's School of Medicine, leading the R-loops & Genome Stability Lab. His research focuses on DNA-RNA hybrid structures (R-loops) at DNA double-strand breaks and their impact on genome stability, with direct implications for neurodevelopmental disorders and cancer therapeutics.
Dr Ngo's academic foundation includes:
- Undergraduate DNA repair research on E. coli mutants at the University of Nottingham under Prof. Robert Lloyd
- D.Phil. studies at the University of Oxford investigating Bloom helicase interactions in budding yeast with Prof. Ian Hickson
His laboratory investigates R-loop dynamics in DNA repair mechanisms using cutting-edge approaches including CRISPR editing, nanopore sequencing, and induced pluripotent stem cells. Current projects examine how R-loop dysregulation contributes to autism spectrum disorder, intellectual disability, and ADHD through UPF1/UPF3B dysfunction, while also exploring PARP inhibition in telomere crisis scenarios for cancer therapy. The multidisciplinary work bridges molecular genetics, neuroscience, and clinical oncology to uncover fundamental genome stability mechanisms.
Analysis of Dr Ngo's publication record (2003-2022) reveals consistent innovation in DNA repair pathways across model systems from archaea to human cells. His work demonstrates conserved mechanisms in homologous recombination regulation, with recent breakthroughs in R-loop formation at double-strand breaks (2021 Nature Communications paper). Key trajectories include telomere maintenance defects, resection control by checkpoint clamps, and the intersection of RNA surveillance with genome stability - establishing foundational knowledge for neurodevelopmental disorder pathogenesis.
Scientific recognition includes:
- Wellcome Trust Career Development Award (2023; £1.8M) for neurodevelopmental disorder research
Dr Ngo actively mentors PhD candidates including Angelos Damo in genome stability and neurodevelopmental disorder research. His laboratory operations are primarily funded by the Wellcome Trust award investigating R-loop roles in neurodevelopment, complemented by prior project grants on telomere crisis and DNA repair mechanisms. The lab maintains strategic collaborations across Cardiff University's neuroscience and oncology divisions.
The R-loops & Genome Stability Lab operates from Cardiff University's Cancer Genetics Building at University Hospital of Wales, comprising Dr. Siamak Kamranvar and Dr. Ashley Parkes. The team employs integrated methodologies including single-molecule PCR, INDUCE-seq DSB mapping, and CRISPR-based genome engineering to dissect R-loop functions in disease contexts.
