Daniel Ladant

Daniel Ladant is a senior researcher at the Institut Pasteur in Paris, France, affiliated with the Unit of Biochemistry of Macromolecular Interactions. He leads multiple research projects focused on the Bordetella pertussis adenylate cyclase (CyaA) toxin, including its structure-function relationships, protein-protein interactions, and use as a vaccine vector. He is a Principal Investigator (PI) on several ongoing studies, working in close collaboration with teams such as Immune Regulation and Vaccinology.

His research interests center on molecular mechanisms of bacterial pathogenesis, particularly protein-protein and protein-membrane interactions. He is a co-developer of the Bacterial Adenylate Cyclase-based Two-Hybrid (BACTH) system, a widely used genetic tool for detecting in vivo protein interactions. His work combines biochemical, biophysical, and genetic approaches to study toxin translocation, membrane binding, and the structural dynamics of RTX proteins. He has made significant contributions to understanding the activation of the CyaA catalytic domain by calmodulin and the calcium-induced folding of RTX motifs.

The recent publications co-authored by Daniel Ladant reflect a strong trend in molecular microbiology, structural biology, and toxinology. A significant portion of his work involves methodological development (e.g., BACTH, B2LiVe NMR method) and the detailed biophysical characterization of bacterial toxins and secretion systems. There is a clear focus on host-pathogen interactions, membrane dynamics, and the structural basis of virulence, with applications in vaccine design and antimicrobial strategies.

Scientific Awards:

• No specific awards mentioned in the provided text.

Advising and Grants:

• Daniel Ladant is listed as Principal Investigator (PI) on multiple ongoing projects at the Institut Pasteur, including: 'In vivo analysis of protein-protein interactions', 'Recombinant CyaA toxin as an innovative vaccine vehicle', and 'Structure-function studies of the CyaA toxin'.
• He has collaborated on research with teams such as C. Leclerc's group in Immune Regulation and Vaccinology.
• While specific grants are not listed, his role as PI on multiple projects and his continued publication record indicate active grant funding.

Labs and Teams:

• He is a key member of the Biochemistry of Macromolecular Interactions Unit at the Institut Pasteur.
• He collaborates closely with Alexandre Chenal's BiophysiCyaA group.
• His research is conducted within a multidisciplinary team environment that includes post-doctoral fellows, PhD students, technicians, and other researchers.