معرفی
Dr. B. Franklin Pugh is a Professor in the Department of Molecular Biology and Genetics at Cornell University, where he leads the Pugh Lab in the Biotechnology Building (467 Biotechnology Building, 526 Campus Rd, Ithaca, NY 14853). His research spans multiple institutions including prior affiliations at UW-Madison, Cal-Berkeley, and Penn State.
His laboratory focuses on uncovering fundamental molecular mechanisms of eukaryotic gene regulation, specifically studying how transcription machinery assembles at promoters and the role of enhancers in accelerating this assembly. The lab employs ultra-high resolution techniques like ChIP-exo to examine protein organization on genomes at single base-pair resolution.
- Yeast Epigenome Project (YEP): Comprehensive analysis of Saccharomyces genome regulation through high-resolution ChIP-exo, published in Nature (Rossi et al., 2021)
- PCRP (Protein Capture Reagent Program): Mapping protein-DNA interactions in human/mouse cell types and diseased specimens
- PEGR (Platform for Epigenetic and Genomic Regulation): Development of computational infrastructure for genomic data management
The Pugh Lab emphasizes diversity of views and cultural backgrounds to foster innovation in understanding genome regulation mechanisms. They maintain several bioinformatics tools including ScriptManager for base-pair resolution analysis of genomic assays.
