Andrew Holding

Dr. Andrew Holding is a Senior Lecturer in Biomedical Science within the Department of Biology at the University of York. His research program centers on steroid hormone receptor dynamics across healthy tissues and cancer pathologies, with particular emphasis on glucocorticoid and estrogen receptors in breast cancer mechanisms. Methodologically, he employs CRISPR/Cas9 genome editing, single-cell RNA sequencing, and machine learning to characterize co-regulator proteins and their roles in treatment resistance.

His core research examines:

• Cellular response mechanisms to steroid hormones
• Computational modeling of receptor interaction networks
• CRISPR-based functional genomics in cancer models
• Machine learning integration of multi-omics data
• Evolution of drug resistance in hormone-driven cancers

Publications show consistent focus on chromatin dynamics, transcriptional regulation in cancer (particularly breast cancer and leukemia), and development of computational tools for genomic analysis. Recent work emphasizes hypoxia pathways, metabolic reprogramming in metastasis, and epigenetic drivers of tumor microenvironments.

Active grants include:

• BBSRC IAA: Establishing tissue differentiation models of human breast epithelium
• MRC-funded project on sodium signaling in tumor hypoxia
• Deep learning integration of interactome data for protein-protein interaction prediction

He leads laboratory investigations into steroid receptor co-regulators and maintains collaborations across computational biology and clinical oncology domains.