Rupert Tscheließnig

Dipl.-Ing. Dr. Rupert Tscheließnig is a researcher at the Institute of Bioprocess Science and Engineering, part of the Department of Biotechnology and Food Science at the University of Natural Resources and Life Sciences, Vienna (BOKU). His work focuses on bioprocess engineering, structural biology, and molecular simulations, particularly in the context of protein purification, bioseparations, and nanoscale phenomena.

• Key Research Areas
  • Protein A and hydrophobic interaction chromatography
  • Structural analysis using Small Angle X-ray Scattering (SAXS)
  • Molecular simulations of adsorption and interfacial phenomena
  • Enzyme conformational dynamics
  • Nanoscale material design for bioseparations
• Selected Projects
  • Lead project on Precisely Patterned Nanofibers for High-Performance Bioseparations (2020–2025, EU-funded)
  • Subproject leader in the FWF-funded Doktoratskolleg Biomolecular Technology of Proteins (2019–2022)
  • Contributed to multiple EC-funded initiatives on protein purification and bioprocess optimization
• Advising
  • Advisor for Valentina Ruocco's 2024 doctoral thesis on N-glycans in IgA2 and SARS-CoV-2 RBD
  • Advisor for Bohuslav Motycka's 2023 work on Cellobiose Dehydrogenase Dynamics

His research combines experimental techniques (SAXS, X-ray scattering) with computational methods to investigate protein-ligand interactions, adsorption mechanisms, and nanoscale structural changes. Publications span journals like Journal of Chromatography A, FEBS Journal, and Biotechnology and Bioengineering, with a focus on translating molecular insights into industrial applications.