معرفی
Brad Rothberg, PhD, is a Professor in the Department of Medical Genetics and Molecular Biochemistry at the Lewis Katz School of Medicine, Temple University. His research is centered on ion channels, particularly calcium-activated potassium channels, using a multidisciplinary approach combining electrophysiology, mutagenesis, spectroscopy, and structural biology.
Research Interests: Dr. Rothberg's work focuses on understanding how ion channels function at the molecular level. His laboratory investigates the structure-function relationships of ion channels, especially BK (Big Potassium) and related channels, which play critical roles in regulating cellular excitability in nerve and muscle cells. These channels are activated by calcium and voltage, making them key integrators of cellular signals. His research addresses fundamental questions such as how calcium binds to the channel, how the gate opens and closes, and how different domains communicate within the protein.
Recent Publications: His recent publications highlight a strong emphasis on calcium signaling, ion channel biophysics, and integrative approaches combining computational and experimental methods. Articles in journals like Nature Reviews Molecular Cell Biology and Journal of General Physiology reflect his leadership in synthesizing complex mechanisms of ion channel regulation, particularly involving STIM proteins, RCK gating rings, and lipid modulation of channel activity. His work bridges molecular detail with physiological relevance.
- Lyndon B. Johnson Research Award from the American Heart Association, Texas affiliate (2002)
Training and Mentorship: Dr. Rothberg has trained in neurobiology at Harvard Medical School and physiology at the University of Miami. He leads a well-equipped laboratory capable of performing patch-clamp electrophysiology, recombinant DNA work, protein purification, and fluorescence spectroscopy. He is actively involved in mentoring students and postdoctoral researchers, though specific advisees are not listed in the provided text.
Laboratory and Techniques: His lab utilizes advanced techniques including site-directed mutagenesis, patch-clamp recording, lipid bilayer experiments, fluorescence microscopy, and X-ray crystallography to dissect ion channel mechanisms. These tools allow for a comprehensive understanding of channel architecture and dynamics.
