Andrei Sivolob

Andrei Sivolob is a Professor in the Department of General and Medical Genetics at the Educational and Scientific Centre 'Institute of Biology and Medicine' of Taras Shevchenko National University of Kyiv. His academic career spans decades, focusing on chromatin structure and dynamics.

• 1977–1982: Master's in Biophysics, Taras Shevchenko National University of Kyiv
• 1985: PhD in Biochemistry, Taras Shevchenko National University of Kyiv
• 2006: Doctor of Science in Biophysics, Taras Shevchenko National University of Kyiv
• 2009: Appointed Professor, Taras Shevchenko National University of Kyiv

Sivolob's research initially centered on nucleosome conformational flexibility, employing fluorescence spectroscopy and theoretical modeling. His recent work explores chromatin loops and their role in transcription regulation through the comet assay, a method for studying DNA damage and organization. This approach has revealed insights into loop domain topology and re-organization in cells of varying types and states.

His publications highlight expertise in electrophoresis, thermodynamic analysis, and biophysical modeling, with papers in journals like Molecular Cell and Biochimica et Biophysica Acta. While no specific awards or students are listed, his ongoing projects aim to characterize chromatin loop organization across cell types.

Technical expertise includes fluorescence spectroscopy, electrophoresis variants, kinetic analysis, theoretical modeling, and programming. A schematics of his comet assay methodology illustrates how kinetic plots help estimate supercoiling levels and loop size distributions.