- Mass Spectrometry
- Proteomics
- Metabolomics
- +۵ مورد دیگر
Dr. Ljiljana (Lili) Paša-Tolić is a distinguished Lab Fellow and Lead Scientist for Visual Proteomics at Pacific Northwest National Laboratory (PNNL), where she works within the Environmental Molecular Sciences Division and the Environmental Molecular Sciences Laboratory (EMSL) user program. She brings world-leading expertise in native-state and top-down proteomics, mass spectrometry, and the Biomolecular Pathways Integrated Research Platform, combining these with Functional Bioimaging and Cell Signaling platforms to develop transformational capabilities for dynamic spatio-temporally resolved proteomic imaging in cells. Shipley Capturing Federal Business Course (2013) Emerging Leader Program (2012) Management Skills Development Program (2003) Postdoc, National High Magnetic Field Laboratory (1995–1997) Postdoc, PNNL (1993–1995) PhD in Chemistry, University of Zagreb (1992) MS in Theoretical and Physical-Organic Chemistry, University of Zagreb (1989) BS in Chemistry, University of Zagreb (1986) Dr. Paša-Tolić specializes in developing sophisticated analytical methods with emphasis on Fourier transform mass spectrometry and micro-separations. Her research focuses on applying these techniques to accurately quantify spatiotemporal changes in protein (metabolite) abundance, identity, and activity. Her work bridges the gap between advanced instrumentation development and biological applications, particularly in environmental systems, microbial communities, and plant-microbe interactions. She has pioneered approaches for single-cell metabolomics, top-down proteomics, and mass spectrometry imaging that have transformed how researchers study complex biological systems at unprecedented resolution. The trend in Dr. Paša-Tolić's recent publications demonstrates her leadership in advancing mass spectrometry technologies while applying them to increasingly complex biological questions. Her work spans environmental science, microbiology, plant biology, and immunology, showing how fundamental advances in analytical methodology can be leveraged across diverse research domains. Key themes include single-cell analysis, interlaboratory standardization, top-down proteomics, and the development of novel instrumentation approaches that push the boundaries of detection sensitivity and spatial resolution. The Analytical Scientist Power List (2019) Spectrometry Global Impact Award (2015) Director's Award, EMSL (2010) Systems Biology Fellows Mentor Award (2007) Outstanding Merit Award, International Immunology (2007) Key Contributor Award, PNNL (2002, 2003) Outstanding Performance Award, PNNL (1998, 1999, 2000) International Institute of Quantum Chemistry and Solid-State Theory Award (1988) As a leader in her field, Dr. Paša-Tolić has served in numerous professional capacities including Treasurer of the American Society for Mass Spectrometry (2020–2022), Editorial Board Member for the Journal of the American Society for Mass Spectrometry (2017–Present), and Member at Large on the Board of Directors for the Consortium for Top-Down Proteomics (2012–Present). She has organized and lectured at the 'Mass Spectrometry in Biology and Medicine' summer school in Dubrovnik, Croatia since 2007, demonstrating her commitment to training the next generation of scientists. Her research has been supported by multiple federal agencies including the Department of Energy, National Institutes of Health, and National Science Foundation. Dr. Paša-Tolić leads a dynamic research team at PNNL focused on visual proteomics, which combines advanced mass spectrometry with imaging techniques to study biological systems at unprecedented resolution. Her group works at the intersection of the Functional and Systems Biology group, the Biomolecular Pathways Integrated Research Platform, and the Functional Bioimaging platform, creating a synergistic environment for innovation. The team has developed novel capabilities for single-cell analysis, top-down proteomics, and mass spectrometry imaging that are being applied to diverse research areas from environmental systems to human health.

