Romain Guiet

Romain Guiet is a Scientist at the BioImaging and Optics Core Facility (BIOP) and a Lecturer in the EDMS program at École Polytechnique Fédérale de Lausanne (EPFL). He is affiliated with the School of Life Sciences (SV) and contributes to EPFL’s imaging community through technical support, teaching, and research. His roles span scientific support, education, and institutional service, including membership in the School Council SV.

His research interests center on BioImage Analysis, with expertise in image acquisition (widefield, confocal, super-resolution), image processing, and quantitative data extraction. He develops open-source workflows using tools like ImageJ/Fiji, QuPath, Python, and R, promoting FAIR data principles. His background includes a PhD in Biology with a focus on immunology, cancer, 3D cell migration, and organoid systems. He supports experimental design and staining strategies for in vitro models including cell cultures and organoids.

His recent publications highlight interdisciplinary work in organoid modeling, cardiogenesis, neural circuit imaging, and bioimage informatics. He co-developed DEVILS, a data visualization tool, and contributes to NEUBIAS training initiatives. His work emphasizes open science and community engagement through platforms like forum.image.sc.

• Metabolic reprogramming in osteoclasts
• Osteoclasts: Other functions
• RANKL-responsive epigenetic mechanism reprograms macrophages into bone-resorbing osteoclasts
• Nuclear receptors in osteoclasts
• WNT-modulating gene silencers as a gene therapy for osteoporosis, bone fracture, and critical-sized bone defects

Romain Guiet advises on microscopy-based projects and mentors students through EPFL’s teaching programs. He is actively involved in grant-supported research and collaborative projects across EPFL. He is a key contributor to the BioImaging and Optics Core Facility, supporting a wide range of life science research. He also participates in the School Council SV, contributing to academic governance.