Nikolaus Virgolini

Nikolaus Virgolini is a researcher at the Institute of Bioprocess Science and Engineering within the Department of Biotechnology and Food Science at the University of Natural Resources and Life Sciences, Vienna (BOKU). He holds a Dipl.-Ing. and Ph.D., with expertise in mammalian cell biotechnology and bioprocess optimization.

His research focuses on

• Cellular response to evolutionary pressures in HEK293 cells
• Characterization of genetic/epigenetic states for biopharmaceutical applications
• Advanced analytics for gene therapy vectors (AAVs)
• Metabolic engineering for sustainable polymer production

Recent publications highlight methodological advances in bioprocess engineering and molecular biotechnology, particularly in

• AAV vector separation techniques
• HEK293 cell line optimization
• Protein glycosylation and clearance mechanisms

He participates in international conferences like ESGCT and PEACe, contributing to knowledge transfer in bioprocessing. Currently involved in the Christian Doppler Laboratory for Knowledge-based Production of Gene Therapy Vectors (2023-2029).