Nicole Borth

Nicole Borth is Associate Professor (associate Univ.Prof.) at the University of Natural Resources and Life Sciences, Vienna (BOKU) and Deputy Head of the Institute of Animal Cell Technology and Systems Biology. Her work sits at the intersection of cell engineering, systems biology and biopharmaceutical manufacturing, with CHO and HEK293 cells as primary platforms.

Research in a nutshell:

• Genome-wide CRISPR/Cas deletion and activation screens to map essential loci and boost recombinant protein titres.
• Epigenetic and synthetic-biology toolboxes (dCas9-DNMT, synthetic promoters, RNA devices) for multiplexed gene-control.
• Glyco-engineering and biomarker discovery to optimise critical quality attributes of monoclonal antibodies.
• Low-cost, animal-component-free media design and microfluidic single-cell cloning to shorten development timelines.

Between 2022-2025 her group released a rapid succession of papers exploiting nanopore Cas9-targeted sequencing to pinpoint transgene integration sites, unveiled novel stress-biomarkers for difficult-to-express mAbs, and provided public-domain glyco-analytics for the NIST CHO reference line. Parallel projects apply similar tool-chains to AAV production in HEK293 and characterise human diamine oxidase biopharmaceuticals.

Awards & funding: Specific prizes not enumerated in supplied text; however, the volume and recency of high-impact publications indicate sustained competitive funding.

Contact: nicole.borth@boku.ac.at | Tel +43 1 47654-79064 | Muthgasse 11, 1190 Vienna, Austria.